Original Publications
2013
Pronovost SM, Beckerle MC, Kadrmas JL (2013) Elevated expression of the Integrin Associated Protein PINCH Suppresses the Defects of Drosophila melanogaster Muscle Hypercontraction Mutants. PLOS Genetics – in press.
2012
Chapin LM, Blankman E, Smith MA, Shiu YT, Beckerle MC (2012) Lateral communication between stress fiber sarcomeres facilitates a local remodeling response. Biophysical Journal 103:2082-92. PMCID3512046.
Chaturvedi A, Hoffman LM, Welm AL, Lessnick SL, Beckerle MC (2012) The EWS/FLI Oncogene Drives Changes in Cellular Morphology, Adhesion, and Migration in Ewing Sarcoma. Genes & Cancer. 3:102-16. PMCID3463921.
Spraker HL, Price SL, Chaturvedi A, Schiffman D, Jones KB, Lessnick SL, Beckerle M, Randall RL (2012) The clone wars—revenge of the metastatic rogue state: The sarcoma paradigm. Front Oncol 2:2. PMCID3355929.
Elias, MC, Pronovost SM, Cahill KJ, Beckerle MC, Kadrmas JL (2012) A critical role for Ras suppressor-1 (RSU-1) revealed when PINCH-Integrin-linked kinase (ILK) binding is disrupted. J. Cell Science. 125:3185-3194. PMCID3434853.
Hoffman, LM, CC Jensen, A Chaturvedi, M Yoshigi, MC Beckerle (2012) Stretch-induced actin remodeling requires targeting of zyxin to stress fibers and recruitment of actin regulators. Mol Biol Cell. 23:1846-59. PMCID3350550.
2011
Beckerle MC, Reed KL, Shafer M-A, Scott RP, Towner D, Valantine HA, Zahniser NR (2011) Medical faculty development: A modern-Day Odyssey. Sci. Transl. Med. 3, 104cm31. PMC Journal – In process.
Clark KA, Lesage-Horton H, Zhao C, Beckerle MC, Swank DM (2011) Deletion of Drosophila muscle LIM protein decreases flight muscle stiffness and power generation. AM J Physiol Cell Physiol. 301:C373-82. PMCID3154547.
2010
Beckerle MC (2010) How cell biologists can contribute to improving cancer outcomes. Mol Biol Cell. 21:3788-9. PMCID2982089.
Smith MA, Blankman E, Gardel ML, Luettjohann L, Waterman CM, Beckerle MC (2010) A zyxin-mediated mechanism for actin stress fiber maintenance and repair. Dev Cell. 19:365-76. PMCID2954498.
Hervy M, Hoffman LM, Jensen CC, Smith MA, Beckerle MC (2010) The LIM protein Zyxin binds CARP-1 and promotes apoptosis. Genes & Cancer. 1:506-15. PMCID2941194.
SJ Mulvihill, MC Beckerle, J Shea, M Firpo, K Boucher, L Emerson, C Scaife (2010) Prognostic significance of PINCH signaling in human pancreatic adenocarcinoma. HPB. 12:352-8. PMCID2951825.
Renfranz PJ, E Blankman, MC Beckerle (2010) The cytoskeletal regulator zyxin is required for viability in Drosophila melanogaster. The Anatomical Record. 293:1455-69. PMCID2939194.
Lilly B, Clark KA, Yoshigi M, Pronovost S, Wu ML, Periasamy M, Chi M, Paul RJ, Yet SF, Beckerle MC (2010) Loss of the Serum Response Factor Cofactor, Cysteine-Rich Protein 1, Attenuates Neointima Formation in the Mouse. Arterioscler Thromb Vasc Biol. 30:694-701. PMC Journal – In Process.
2009
Han HF and MC Beckerle (2009) The ALP-Enigma protein ALP-1 functions in actin filament organization to promote muscle structural integrity in Caenorhabditis elegans. Mol Biol Cell 20:2361-70. PMCID2675616.
Chastre E, M Abdessamad, A Kruglov, E Bruyneel, M Bracke, Y Di Gioia, MC Beckerle, F van Roy, L Kotelevets (2009) TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness. FASEB J. 23:916-28. PMC Journal – In Process.
2008
Hansen MD, and MC Beckerle (2008) alpha-Actinin links LPP, but not zyxin, to cadherin-based junctions. Biochem Biophys Res Commun. 371:144-8. PMCID2676570.
Mery A, O Taghli-Lamallem, KA Clark, MC Beckerle, X Wu, K Ocorr, R Bodmer (2008) The Drosophila muscle LIM protein, Mlp84B, is essential for cardiac function. J Exp Biol 211:15-23.
2007
Kadrmas, JL, MA Smith, SM Pronovost, and MC Beckerle (2007) Characterization of RACK1 function in Drosophila development. Dev Dyn 236:2207-15.
Clark, K.A., J.M. Bland, and M.C. Beckerle (2007) The Drosophila muscle LIM proten, Mlp84B, cooperates with D-titin to maintain muscle structural integrity. J Cell Sci. 120:2066-77.
Pomies, P., M. Pashmforoush, C. Vegezzi, K.R. Chien, C. Auffray, and M.C. Beckerle (2007) The cytoskeleton-associated PDZ-LIM protein, ALP, acts on serum response factor activity to regulate muscle differentiation. Mol Biol Cell 18(5):1723-33.
2006
Hervy, M., L. Hoffman, and M.C. Beckerle (2006) From the membrane to the nucleus and back again: bifunctional focal adhesion proteins. Curr Opin Cell Biol. 18(5), 524-32.
Hansen, M.D., and M.C. Beckerle (2006) Opposing roles of zyxin/LPP ACTA repeats and the LIM domain region in cell-cell adhesion. J Biol Chem. 281:16178-88.
Takizawa, N., T.C. Smith, T. Nebl, J.L. Crowley, S.J. Palmeiri, L.M. Lifshitz, A.G. Ehrhardt, L.M. Hoffman, M.C. Beckerle, and E.J. Luna (2006) Supervillin modulation of focal adhesions involving TRIP6/ZRP-1. J Cell Biol 174(3): 447-58.
Hoffman, L.M., C.C. Jensen, S. Kloeker, C.-L.A. Wang, M. Yoshigi, and M.C. Beckerle (2006) Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling. J Cell Biol 172:771-82.
McKeown C.R., H.F. Han, and M.C. Beckerle (2006) Molecular characterization of the Caenorhabditis elegans ALP/Enigma gene alp-1. Dev Dyn 235(2):530-8.
2005
Yoshigi, M., L.H. Hoffman, C.C. Jensen, H.J. Yost, and M.C. Beckerle (2005) Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement. J Cell Biol 171(2):209-15.
Kato, T., J. Muraski, Y. Chen, Y. Tsujita, J. Wall, C.C. Glembotski, E. Schaefer, M.C. Beckerle, and M.A. Sussman (2005) Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt. J Clin Invest 115-:2716-30.
Norman, K.R., R.T. Fazzio, J.E. Mellem, M.V. Espelt, K. Strange, M.C. Beckerle, and A.V. Maricq (2005) The Rho/Rac family guanine exchange factor VAV-1 regulates rhythmic behaviors in C. elegans. Cell 123(1):119-32.
2004
Kadrmas, J.L., M.A. Smith, K.A. Clark, S.M. Pronovost, N. Muster, J.R. Yates, III, M.C. Beckerle (2004) The integrin effector PINCH regulates JNK activity and epithelial migration in concert with Ras suppressor 1. J Cell Biol 167(6):1019-24.
Kadrmas, J.L., and M.C. Beckerle (2004) The LIM domain: from the cytoskeleton to the nucleus. Nature Reviews Molecular Cell Biology 5:920-931.
Kloeker, S., M.B. Major, D.A. Calderwood, M.H. Ginsberg, D.A. Jones, M.C. Beckerle (2004) The Kindler syndrome protein is regulated by TGFbeta and involved in integrin-mediated adhesion. J Biol Chem 279(8):6824-33.
2003
Gorenne I, R.K. Nakamoto, C.P. Phelps, M.C. Beckerle, A.V. Somlyo, A.P. Somlyo (2003) LPP, a LIM protein highly expressed in smooth muscle. Am J Physiol Cell Physiol 285(3):C674-85.
Clark, K.A., M. McGrail, M.C. Beckerle. (2003) Analysis of PINCH function in Drosophila demonstrates its requirement in integrin-dependent cellular processes. Development 130:2611-21.
Chang, D.F., N.S. Belaguli, D. Iyer, W.B. Roberts, S.P. Wu, X.R. Dong, J.G. Marx, M.S. Moore, M.C. Beckerle, M.W. Majesky, R.J. Schwartz (2003) Cysteine-Rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors. Dev Cell. 4:107-18.
Renfranz, P.J., S.E. Siegrist, B.E. Stronach, T. Macalma, M.C. Beckerle. (2003) Molecular and phylogenetic characterization of Zyx102, a Drosophila orthologue of the Zyxin family that interacts with Drosophila Enabled. Gene. 305:13-26.
Henderson, J. R., M.C. Beckerle, P. Pomies. (2003) ALP and MLP distribution during myofibrillogenesis in cultural cardiomyocytes. Cell Mot & Cytoskeleton. 54:254-65.
Hoffman, L.M., D.A. Nix, B. Benson, R. Boot-Hanford, E. Gustafsson, C. Jamora, A.S. Menzies, K.L. Goh, C.C. Jensen, F.B. Gertler, E. Fuchs, R. Faessler, M.C. Beckerle. (2003) Targeted disruption of the murine zyxin gene. Mol. & Cell. Biol. 23:70-79.
2002
Clark, K.A., A.S. McElhinney, M.C. Beckerle, C.C. Gregorio. (2002) Striated muscle cytoarchitecture: An intricate web of form and function. Annu Rev Cell Dev Biol. 18:637-706.
Yi, J., S. Kloeker., C.C. Jensen, S. Bockholt, H. Honda, H. Hirai, M.C. Beckerle. (2002) Members of the zyxin family of LIM proteins interact with members of the p130cas family of signal transducers. J. Biol. Chem. 277:9580-9589.
Renfranz, P.J. and M.C. Beckerle. (2002) Doing (F/L)PPPPs: EVH1 domains and their proline-rich partners in cell polarity and migration. Curr. Opinion Cell Biol. 14:88-103.
Henderson, J.R., D. Brown, J.A. Richardson, E.N. Olson, and M.C. Beckerle. (2002) Expression of the gene encoding the LIM protein CRP2: A developmental profile. J. Histochem. Cytochem. 50:107-12.
2001
Lilly, B., E.N. Olson, M.C. Beckerle. (2001) Identification of a CArG box-dependent enhancer within the cysteine-rich protein 1 gene that directs expression in arterial but not venous and visceral smooth muscle cells. Dev. Biol. 240:531-547.
Nix, D., Fradelizi, S. Bockholt, B. Menichi, D. Louvard, E. Friederich, and M.C. Beckerle. (2001) Targeting of zyxin to sites of actin membrane interaction and to the nucleus. J. Biol. Chem. 276:34759-34767.
Pashmforoush, M., P. Pomies, K.L. Peterson, S. Kobalak, J. Ross, A. Hefti, U. Aebi, M.C. Beckerle and K. R. Chien. (2001) Adult mice deficient in actinin-associated LIM domain protein reveal a developmental pathway for adult right ventricular cardiomyopathy. Nature Medicine 7:591-597.
2000
Neufeld, K.L., D.A. Nix, H. Bogerd, Y. Kang, M.C. Beckerle, B.R. Cullen, and R.L. White. (2000) Adenomatous polyposis coli protein contains two nuclear export signals and shuttles between the nucleus and cytoplasm. Proc. Natl. Acad. Sci. USA 97:12085-90.
Drees, B., E. Friederich, J. Fradelizi, D. Louvard, M.C. Beckerle, R.M. Golsteyn. (2000) Characterization of the interaction between zyxin and Ena/VASP family of proteins: Implications for actin cytoskeleton organization. J. Biol. Chem. 275:22503-22511.
Harper, B.D, M.C. Beckerle, and P. Pomies. (2000) Fine mapping of the α-actinin binding site within cysteine-rich protein. Biochem. J. 350:269-274.
1999
Drees, B.E., K. Andrews, and M.C. Beckerle. (1999) Molecular dissection of zyxin function reveals its involvement in cell motility. J. Cell Biol. 147:1549-1559.
Zhao, M.K., Y. Wang, K. Murphy, J. Yi, M.C. Beckerle, and T.D. Gilmore. (1999) LIM domain-containing protein Trip6 can act as a co-activator for the v-Rel transcription factor. Gene Expression 8:207-217.
Pomies, P., T. Macalma, and M.C. Beckerle. (1999) Purification and characterization of an α-actinin-binding PDZ-LIM protein that is up-regulated during muscle differentiation. J. Biol. Chem. 274:29242-29250.
Stronach, B.E., P.J. Renfranz, B. Lilly, and M.C. Beckerle. (1999) Muscle LIM proteins are associated with muscle sarcomeres and require dMEF2 for their expression during Drosophila myogenesis. Mol. Biol. Cell 10:2329-2342.
Yao, X., G.C. Perez-Alvarado, H.A. Louis, P. Pomies, C. Hatt, M.F. Summers, and M.C. Beckerle. (1999) Solution structure of the chicken cysteine-rich protein, CRP1, a double-LIM protein implicated in muscle differentiation. Biochemistry 38:5701-5713.
Fradelizi, J., E. Friederich, D. Louvard, M.C. Beckerle, and R.M. Golsteyn. (1999) Quantitative measurements of proteins by Western blotting with Cy5TM-coupled secondary antibodies. Biotech. 26:484-494.
Hobert, O., D.G. Moerman, K.A. Clark, M.C. Beckerle, and G. Ruvkun (1999) A conserved LIM protein that affects muscular adherens junction integrity and mechanosensory function in Caenorhabditis elegans. J. Cell Biol. 144:45-57.
Henderson, J.R., T. Macalma, D. Brown, J.A. Richardson, E.N. Olson, and M.C. Beckerle. (1999) The LIM protein, CRP1, is a smooth muscle marker. Dev. Dynamics 214:229-238.
1998
Beckerle, M.C. (1998) Spatial control of actin assembly: Lessons from Listeria. Cell 95:741-748.
Ahern-Djamali, S.M., A.R. Comer, C. Bachmann, M.C. Beckerle, U. Walter, and F.M. Hoffmann. (1998) Mutations in Drosophila enabled and rescue by human vasodilator-stimulated phosphoprotein (VASP) indicate important functional roles for Ena/VASP homology domain 1 (EVH1) and EVH2 domains. Mol. Biol. Cell 9:2157-2171.
Schmeichel, K.L. and M.C. Beckerle. (1998) The LIM domains of cysteine-rich protein 1 (CRP1) are essential for its zyxin-binding function. Biochem. J. 331:885-892.
Yi, J. and M.C. Beckerle. (1998) The human TRIP6 gene encodes a LIM domain protein and maps to chromosome 7q22, a region associated with tumorigenesis. Genomics 49:314-316.
Schmeichel, K.L., B.E. Stronach, and M.C. Beckerle. (1998) Purification and assay of zyxin. Methods Enzymol. 298:62-76.
1997
Louis, H.A., J. Pino, K.S. Schmeichel, P. Pomies, and M.C. Beckerle. (1997) Comparison of three members of the cysteine-rich protein family reveals functional conservation and divergent patterns of gene expression. J. Biol. Chem. 272:27484-27491.
Golsteyn, R.M., M.C. Beckerle, T. Koay, D. Louvard, and E. Friederich. (1997) Structural and functional similarities between the human cytoskeletal protein zyxin and the ActA protein of Listeria monocytogenes. J. Cell Sci. 110:1893-1906.
Pomies, P., H.A. Louis, and M.C. Beckerle. (1997) CRP1, a LIM domain protein implicated in muscle differentiation, interacts with α-actinin. J. Cell Biol. 139:157-168.
Nix, D. and M.C. Beckerle. (1997) Nuclear-cytoplasmic shuttling of the focal contact protein, zyxin: A potential mechanism for communication between sites of cell adhesion and the nucleus. J. Cell Biol. 138:1139-1147.
Beckerle, M.C. (1997) Zyxin: zinc fingers at sites of cell adhesion. Bioessays 19:949-957.
Schmeichel, K.L. and M.C. Beckerle. (1997) Molecular dissection of a LIM domain. Mol. Biol. Cell 8:219-230.
1996
Macalma, T., J. Otte, M.E. Hensler, S.M. Bockholt, H.A. Louis, M. Kalffe-Suske, K.H. Grzeschik, D. von der Ahe, and M.C. Beckerle. (1996) Molecular characterization of human zyxin. J. Biol. Chem. 271:31470-31478.
Stronach, B.E., S.E. Siegrist, and M.C. Beckerle. (1996) Two muscle-specific LIM proteins in Drosophila. J. Cell Biol. 134:1179-1195.
Hobert, O., J.W. Schilling, M.C. Beckerle, A. Ullrich, and B. Jallal. (1996) SH3 domain-dependent interaction of Vav with the LIM domain protein, zyxin. Oncogene 12:1577-1581.
Perez-Alvarado, G.C., J.L. Kosa, H.A. Louis, M.C. Beckerle, D.R. Winge, and M.F. Summers. (1996) Structure of the cysteine-rich intestinal protein, CRIP. J. Mol. Biol. 257:153-174.
1995
Weiskirchen, R., J.D. Pino, T. Macalma, K. Bister, and M.C. Beckerle. (1995) The cysteine-rich protein family of highly related LIM domain proteins. J. Biol. Chem. 270:28946-28954.
1994
Bertagnolli, M.E. and M.C. Beckerle. (1994) Regulated membrane-cytoskeleton linkages in platelets. Ann. New York Acad. Sci. 714:88-100.
Schmeichel, K.L. and M.C. Beckerle. (1994) The LIM Domain is a modular protein-binding interface. Cell 79:211-19.
Perez-Alvarado, G.C., C. Miles, J.W. Michelsen, H.A. Louis, D.R. Winge, M.C. Beckerle, and M.F. Summers (1994) Structure of the carboxy-terminal LIM domain from the cysteine rich protein CRP. Nat. Struct. Biol. 1:388-98.
Michelsen, J.W., A.K. Sewell, H.A. Louis, J.I. Olsen, D.R. Davis, D.R. Winge, and M.C. Beckerle. (1994) Mutational analysis of the metal sites in an LIM domain. J. Biol. Chem. 269:11108-13.
Kosa, J.L., J.W. Michelsen, H.A. Louis, J.I. Olsen, D.R. Davis, M.C. Beckerle, and D.R. Winge. (1994) Common metal ion coordination in LIM domain proteins. Biochemistry 33:468-477.
Crawford, A.W., J. Pino, and M.C. Beckerle. (1994) Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeleton. J. Cell Biol. 124:117-127.
1993
Bertagnolli, M.E., S.J. Locke, M.E. Hensler, P.F. Bray, and M.C. Beckerle. (1993) Talin distribution and phosphorylation in thrombin-activated platelets. J. Cell Sci. 106:1189-1199.
Bertagnolli, M.E. and M.C. Beckerle. (1993) Evidence for selective association of a subpopulation of GPIIb-IIIa integrin with the actin cytoskeletons of thrombin-activated platelets. J. Cell Biol. 121:1329-1342.
Michelsen, J.W., K.L. Schmeichel, M.C. Beckerle, and D.R. Winge. (1993) The LIM motif defines a specific zinc-binding protein domain. Proc. Natl. Acad. Sci. USA 90:4404-4408.
1992
Sadler, I., A.W. Crawford, J. Michelsen, and M.C. Beckerle. (1992) Zyxin and cCRP: Two interactive LIM domain proteins associated with the cytoskeleton. J. Cell Biol. 119:1573-1588.
Crawford, A.W., J.W. Michelsen, and M.C. Beckerle. (1992) An interaction between zyxin and α-actinin. J. Cell Biol. 116:1381-1393.
1991
Crawford, A.W. and M.C. Beckerle. (1991) Purification and characterization of zyxin, an 82,000-Dalton component of adherens junctions. J. Biol. Chem. 266:5847-5853.
1990
Beckerle, M.C. and R.K. Yeh. (1990) Talin: Role at sites of cell-sub¬stratum adhesion. Cell Motil. Cytoskel. 16:7-13.
Beckerle, M.C. (1990) The adhesion plaque protein, talin, is phosphory¬lated in vivo in chicken embryo fibroblasts exposed to the tumor promoting phorbol ester. Cell Regulation 1:227-236.
1989
Beckerle, M.C., D.E. Miller, M.E. Bertagnolli, and S.J. Locke. (1989) Activation-dependent redistribution of the adhesion plaque protein, talin, in intact human platelets. J. Cell Biol. 109:3333-3346.
1988
Drenckhahn, D., M.C. Beckerle, K. Burridge, and J. Otto. (1988) Identifica¬tion and subcellular location of talin in various cell types and tissues by means of [125I]vinculin overlay, immunoblotting and immunocyto¬chemistry. Eur. J. Cell Biol. 46:513-522.
1987
Beckerle, M.C., K. Burridge, G. de Martino, and D.E. Croal. (1987) Colocalization of calcium-dependent protease II and one of its sub¬strates at sites of cell adhesion. Cell 51:569-577.
1986
Beckerle, M.C. (1986) Identification of a new protein localized at sites of cell-substrate adhesion. J. Cell Biol. 103:1679-1687.
Horwitz, A.F., K. Duggan, C. Buck, M.C. Beckerle, and K. Burridge. (1986) Interaction of plasma membrane fibronectin receptor with talin--a transmembrane link. Nature 320:531-533.
Beckerle, M.C., T. O'Halloran, and K. Burridge. (1986) Demonstration of a rela¬tionship between talin and P235, a major substrate of the Calcium-dependent protease in platelets. J. Cell. Biochem. 30:259-270.
Sealock, R., B. Paschal, M.C. Beckerle, and K. Burridge. (1986) Talin is a post-¬synaptic component of the rat neuromuscular junction. Exp. Cell Res. 163:143-150.
1985
O'Halloran, T., M.C. Beckerle, and K. Burridge. (1985) Identification of talin as a major cytoplasmic protein implicated in platelet activa¬tion. Nature 317:449-451.
1984
Beckerle, M.C. (1984) Microinjected fluorescent polystyrene beads exhibit saltatory motion in tissue culture cells. J. Cell Biol. 98:2126-2132.
1983
Porter, K.R., M.C. Beckerle, and M.A. McNiven. (1983) The cytoplasmic matrix. Mol. Cell Biol. 2:259-302.
Beckerle, M.C. and K.R. Porter. (1983) Analysis of the roles of micro¬tubules and actin in erythrophore intracellular motility. J. Cell Biol. 96:354-362.
1982
Beckerle, M.C. and K.R. Porter. (1982) Inhibitors of dynein activity block intracellular transport in erythrophores. Nature 295:701-703.
Books, book chapters
Drees, B. and M.C. Beckerle. (1999) Zyxin. In: Guidebook to the Extracellular Matrix, Anchor, and Adhesion Proteins. Oxford University Press.
Pomies, P. and M.C. Beckerle. (1999) The cysteine-rich protein family. In: Guidebook to the Extracellular Matrix, Anchor, and Adhesion Proteins. Oxford University Press.
Schmeichel, K.L., M.C. Beckerle, and A.W. Crawford. (1998) Blot Overlay Assay: A Method to Detect Protein-Protein Interactions. Cell Biology: A Laboratory Handbook Second Edition. Vol. 4 441-449.
Crawford, A.W. and M.C. Beckerle. (1989) Cell-substratum adhesion: Mechanism and regulation. In: Primers in Developmental Biology, Vol. IV. Cyto¬plasmic Organization in Development. George M. Malacinski (ed.). Macmillan Publishing Co.
Porter, K.R., M.C. Beckerle, and M.A. McNiven. (1983) A mechanism for intracellular transport. In: Ramon y Cajal's Contribution to the Neurosciences. Grisolen, Guerri, Samson, Norton and Reinoso-Suarez (eds.).
Byers, H.R., K. Fujiwara, M.C. Beckerle and K.R. Porter. (1980) Micro¬tubule organization and function in chromatophores and scleroblasts in vitro and in situ. In: Microtubules and Microtubule Inhibitors. DeBrabander and DeMey (eds.), Janssen Pharmaceutical Research Foundation, Elsevier/North Holland, Amsterdam.
